Etiological surveillance of mosquito-borne pathogens in Wanzhou district of Chongqing from 2021 to 2023
Objective To investigate the situation of mosquito-borne pathogens carried by mosquitoes in Wanzhou district of Chongqing,and provide reference for the prevention and control of mosquito-borne diseases.Methods From 2021 to 2023,mosquitoes were collected from residential and living areas from June to July every year,and a group of 20-30 mosquitoes of the same species were mixed as one pool to extract nucleic acid.The qRT-PCR method were used to detect dengue virus,yellow fever virus,Japanese encephalitis virus,West Nile virus,Zika virus,chikungunya virus and Sindbis virus.Results From 2021 to 2023,a total of 16 651 mosquitoes(601 groups),including Anopheles sinensis,Aedes albopictus,Culex tritaeniorhynchus and Armigeres subalbatus,were collected for mosquito-borne pathogens detection in Wanzhou district of Chongqing.Sixteen groups of Culex tritaeniorhynchus and Armigeres subalbatus were detected positive of Japanese encephalitis virus with batch positive rate of 2.66％and minimum infection rate of 0.96‰.From 2021 to 2023,Japanese encephalitis virus was detected every year,and there were no statistically significant differences in its batch positive rates and minimum infection rates among different years(all P＞0.05).Moreover,in 2021 and 2022,Japanese encephalitis virus was only detected in Culex tritaeniorhynchus,while in 2023,it was only detected in Armigeres subalbatus.Conclusions Wanzhou district of Chongqing might have formed a natural focus of Japanese encephalitis virus.The control of mosquitoes and health education should be strengthened,so as to reduce the risk of Japanese encephalitis and other mosquito-borne diseases.
万方数据
维普
