Effect of ethanol extract of Anacyclus pyrethrum root on improving airway inflammatory factors and its correlation with intestinal flora in rats with cough variant asthma
Objective To investigate the effect of ethanol extract of Anacyclus pyrethrum root(EEAP)on airway inflammation in rats with cough variant asthma(CVA)and its correlation with intestinal flora.Methods Forty rats with CVA were established and randomly divided into CVA model group,EEAP low-dose group(160 mg/kg),EEAP medium-dose group(320 mg/kg),EEAP high-dose group(640 mg/kg),and positive drug group(prednisone acetate,2.50 mg/kg),with 8 rats in each group;SD rats without modeling were taken as control group.After continuous intervention for 30 days according to the grouping,bronchoalveolar lavage fluid,lung tissue and feces of rats in each group were collected.The levels of interleukin-4(IL-4),IL-13,IL-17 and interferon-γ(IFN-γ)in bronchoalveolar lavage fluid were detected by ELISA;the intestinal flora of rats was detected by high-throughput sequencing,and the expressions of hypoxia-inducible factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF)in lung tissue of rats were detected by Western blotting.Results There were significant differences in IL-4,IL-13,IL-17 and IFN-γ levels in alveolar lavage fluid of rats in each groups(F=63.832,11.869,11.266,4.028;all P＜0.05).Compared with the model group,the levels of IL-4,IL-13,and IL-17 in bronchoalveolar lavage fluid of rats in the medium-dose,high-dose,and positive drug groups of EEAP were significantly decreased,while the level of IFN-γ in bronchoalveolar lavage fluid of rats in the high-dose and positive drug groups of EEAP was significantly increased(all P＜0.05).There were significant differences in HIF-1α and VEGF protein expression in lung tissues of rats in each groups(F=55.898,26.835;all P＜0.05).Compared with the model group,the expressions of HIF-1α and VEGF proteins in lung tissue of rats in the medium-dose,high-dose,and positive drug groups of EEAP were significantly decreased(all P＜0.05).Spearman correlation analysis showed that Prevotellaceae_NK3B31_group were negatively correlated with IL-4,IL-13,and IL-17 levels(r=-0.605,-0.460,-0.522;all P＜0.05),and positively correlated with IFN-γ(r=0.397,P=0.025).Conclusion EEAP could inhibit the expression of bronchial inflammatory factors IL-4,IL-13,and IL-17 in CVA rats,and was correlated with the differential flora that regulate the intestine,which preliminarily suggests that EEAP might be participate in the intestinal-lung axis to improve CVA.
Ethanol extract of Anacyclus pyrethrum rootCough variant asthmaAirway inflammationIntestinal flora
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