Detection of Ki-67,HER-2,β-catenin,P53 protein expression in breast cancer tissues and its relationship with HPV infection
Objective To investigate the expression levels of proliferating cell nuclear antigen Ki-67(Ki-67),human epidermal growth factor receptor 2(HER-2),β-catenin and P53 protein in breast cancer tissues and their relationship with human papillomavirus(HPV)infection.Methods A total of 112 breast cancer patients treated in the Nanyang Central Hospital from September 2020 to June 2023 were selected.Breast cancer tissues were taken as research objects,and normal breast tissues(＞2 cm from the edge of the cancer)were selected as controls.Immunohistochemistry was used to detect the expression of Ki-67,HER-2,β-catenin,and P53 proteins.Second-generation capture hybridization was used to detect HPV infection.The expression of Ki-67,HER-2,β-catenin,and P53 proteins in breast cancer tissues and normal breast tissues,as well as the differences in Ki-67,HER-2,β-catenin,P53 proteins,and HPV infection in breast cancer tissues with different clinical pathological characteristics were analyzed.Results The positive expression rates of Ki-67,HER-2,β-catenin,and P53 proteins in breast cancer tissues were significantly higher than those in normal breast tissues(x2=118.050,40.840,66.878,38.615;all P＜0.05).The positive expression rates of Ki-67,HER-2,β-catenin,and P53 proteins in poorly differentiated breast cancer tissues were significantly higher than those in moderately and highly differentiated breast cancer tissues(x2=12.836,18.532,13.221,13.197;all P＜0.05).The positive expression rates of Ki-67,HER-2,β-catenin,and P53 proteins in TNM stage Ⅲ-Ⅳ breast cancer tissues were significantly higher than those in stage Ⅰ-Ⅱ breast cancer tissues(x2=10.324,24.446,17.437,11.951;all P＜0.05).The positive expression rates of Ki-67,HER-2,β-catenin,and P53 proteins in breast cancer tissues with tumor diameter ≥ 2 cm were significantly higher than those in breast cancer tissues with tumor diameter＜2 cm(x2=28.756,9.006,23.083,13.079;all P＜0.05).The positive expression rates of Ki-67,HER-2,β-catenin,and P53 proteins in breast cancer tissues with lymph node metastasis were significantly higher than those in breast cancer tissues without lymph node metastasis(x2=8.848,29.206,16.254,11.935,all P＜0.05).There were 35 cases of HPV infection,with an infection rate of 31.3％,including 23 cases of HPV 16 infection and 12 cases of HPV 18 infection;the HPV infection rate of poorly differentiated breast cancer tissue was significantly higher than that of moderately and highly differentiated breast cancer tissue(x2=20.970,P＜0.05).The HPV infection rate of breast cancer tissue with lymph node metastasis was significantly higher than that of breast cancer tissue without lymph node metastasis(x2=21.759,P＜0.05).The positive expression rates of Ki-67,HER-2,β-catenin,and P53 proteins in breast cancer tissue with HPV infection were significantly higher than those in breast cancer tissue without HPV infection(x2=11.836,22.414,8.612,20.684;all P＜0.05).Conclusions The up-regulated expression of Ki-67,HER-2,β-catenin and P53 proteins in breast cancer tissues was associated with the degree of differentiation,TNM stage,tumor diameter and lymph node metastasis.HPV infection could increase the expression of Ki-67,HER-2,β-catenin and P53 proteins.
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