Relationship between the expression of TGF-β1,miR-146a in peripheral blood and TFF1,GKN2 in gastric mucosa and the severity of chronic gastritis infected with Hp
Objective To explore the relationship between the expression of transforming growth factor-β1(TGF-β1),miR-146a and gastric mucosa trefoil factor 1(TFF1),gastrokine 2(GKN2)in peripheral blood and the severity of chronic gastritis infected by Helicobacter pylori(Hp).Methods Sixty-two patients with Hp-infected chronic gastritis admitted to the Seventh People's Hospital of Zhengzhou from November 2019 to November 2022 were selected as the case group,and 62 patients with chronic gastritis who received treatment in the hospital and were not infected with Hp were selected as the control group.The expression of TGF-β1,miR-146a and TFF1,GKN2 in peripheral blood and gastric mucosa of the two groups of patients were analyzed.The case groups were grouped according to the operative link on gastritis assessment(OLGA)/operative link on gastritis intestinal metaplasia assessment(OLGIM)stage:patients in stage 0-Ⅱ were included in the mild group,and the severe group in stage Ⅲ-Ⅳ.The differences in TGF-β1,miR-146a expression,TFF1,GKN2 expression and their mRNA levels were explored.Results Compared with control group,the relative expression levels of TGF-β1 and miR-146a in peripheral blood of patients in case group were increased(t=-7.475,-6.883;all P＜0.05).The positive rate of gastric mucosa TFF1,GKN2 in case group was lower than those in control group,and the mRNA level of TFF1,GKN2 in case group was lower than those in control group(x2/t=4.212,4.146,13.760,18.236;all P＜0.05).Compared with the mild group,the relative expression levels of TGF-β1 and miR-146a in peripheral blood of the severe group were increased(t=-10.392,-24.192;all P＜0.05).The positive rate of TFF1,GKN2 in gastric mucosa in severe group was lower than those in mild group,and the mRNA level of TFF1,GKN2 in mild group was lower than those in mild group(x2/t=4.353,5.035,7.398,6.302;all P＜0.05).The area under the curve(AUC)values of the receiver operating characteristic(ROC)curves of TGF-β1,miR-146a relative expression levels,and TFF1/GKN2 mRNA levels in gastric mucosa in severe group were 0.817,0.761,0.776 and 0.838,respectively,and the AUC value of the combined diagnosis of these indexes was 0.900,which was higher than that of the separate diagnosis of each index(P＜0.05).Conclusion In patients with chronic gastritis complicated with Hp infection,the expression levels of TGF-β1 and miR-146a were increased,while the positive expression rate of TFF1,GKN2 gene and its relative mRNA expression level were decreased,which was related to the severity of the disease and had certain diagnostic value.
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