Relationship between microsatellite instability and clinical pathological characteristics of colorectal cancer patients in Yunnan
Objective To analyze the relationship between tissue microsatellite instability(MSI)and clinicopathological characteristics of patients with colorectal cancer,and explore the detection of nucleotides in the 2B3D Panel(BAT-25,BAT-26,D2S123,D5S346 and D17S250).Methods A retrospective cohort study was used to collect the clinicopathological data of 297 patients with colorectal cancer who underwent MSI testing at the Molecular Diagnosis Center of Yunnan Cancer Hospital from July 2022 to April 2023,including 80 cases of left colon cancer,51 cases of right colon cancer,and 166 cases of rectal cancer;33 cases of stage Ⅰ,63 cases of stage Ⅱ,98 cases of stage Ⅲ,51 cases of stage Ⅳ,and 52 cases of uncertain stage;249 cases of Han nationality and 48 cases of non-Han nationality.The microsatellite status of tumor specimens was detected by polymerase chain reaction(PCR)-capillary electrophoresis with patient blood as the control sample,and the patients were divided into MSI-high(MSI-H)group and microsatellite stability(MSS)/MSI-low(MSI-L)group according to the test results.Results Among 297 patients with colorectal cancer,35 were MSI-H(11.8％),6 were MSI-L(2.0％),and 256 were MSS(86.2％).There were significant differences in age,site of onset,family history of colorectal cancer,and tumor N stage between MSI-H and MSS/MSI-L patients(x2=5.188,15.044,43.157,14.261;all P＜0.05),but no significant differences in gender,ethnicity,clinical stage,and tumor M stage(x2=0.346,0.656,1.232,0.012;all P＞0.05).Among the MSI-H loci,the detection rates of single-nucleotide loci were similar(BAT26 91.43％,BAT25 97.14％),and were greater than those of dinucleotide loci D5S346(51.43％),D17S250(60.00％),and D2S123(77.14％).Among the 6 cases of MSI-L,the dinucleotide sites D17S250 and D2S123 were detected in 3 cases each.Conclusions MSI-H patients in Yunnan Province had more special clinicopathological characteristics compared with MSS/MSI-L patients.In the 2B3D Panel,the detection rate of single nucleotides in patients with MSI-H was higher,and the detection rate of dinucleotides varied.
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