The differential expression and significance of mitochondrial DNA haplotype groups in dermatomyositis
Objective To explore the haplotype groups and clinical significance of mitochondrial DNA(mtDNA)in peripheral blood of patients with dermatomyositis.Methods A total of 40 patients with dermatomyositis admitted to the First Affiliated Hospital of Hebei North University from January 2020 to December 2021 were selected as the observation group,and 40 healthy subjects who underwent physical examinations during the same period were selected as the control group.Peripheral blood was collected from the two groups of subjects and mtDNA was extracted.The general data of the two groups were analyzed,and the mitochondrial DNA sequences were amplified by real-time fluorescence quantitative PCR technology to find mutation sites in the D-loop region and preliminarily divide haplotype groups.Logistic regression was used to analyze the relationship between dermatomyositis and haplotype groups.SPSS 23.0 was used for statistical analysis.Results There were no statistically significant differences in gender,age,and immune function between the two groups(all P＞0.05).The relative expression of serum mtDNA in the observation group was lower than that in the control group,and the difference was statistically significant(t=5.121,P＜0.001).The mtDNA haplotype groups of the samples were haplotype groups A,B,M7,and F1;among them,haplotype group M7 might be an independent risk factor for dermatomyositis(P=0.003,OR=2.28).Conclusion mtDNA haplotype group M7 might be closely related to the susceptibility and development of dermatomyositis.
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