Effect of hypoxic culture on embryo development and pregnancy outcome in ICSI
Objective To investigate the effects of different oxygen concentration on the developmental potential and pregnancy outcomes of intracytoplasmic sperm injection(ICSI)embryos.Methods The clinical data of patients who underwent ICSI treatment in the Reproductive Medicine Center of Women and Children's Medical Center Affiliated to Guangzhou Medical University from January 2019 to June 2023 were retrospectively analyzed.According to the different oxygen concentrations in the incubator,they were divided into a hypoxic culture group(5％oxygen concentration)and a hyperoxic culture group(20％oxygen concentration).The developmental potential and pregnancy outcomes of embryos in culture environments with different oxygen concentrations were compared.Results The D3 available embryo rate,D3 high-quality embryo rate,blastocyst formation rate,available blastocyst formation rate,and live birth rate in the hyperoxic culture group were lower than those in the hypoxic culture group,and the differences were statistically significant(x2=101.142,19.390,32.329,38.730,4.598;all P＜0.05).There were no significant differences in fertilization rate,normal fertilization rate,cleavage rate,clinical pregnancy rate of fresh cycle transfer,implantation rate,number of transferred embryos between the hyperoxic culture group and the hypoxic culture group(x2/t=0.280,2.424,3.000,2.512,2.466,-0.468;all P＞0.05).Conclusions Hypoxic culture(5％oxygen concentration)could improve the developmental potential of ICSI fertilized embryos,increase the live birth rate,and obtain more usable embryos.However,these observations should be verified by larger sample size and prospective randomized controlled studies.
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