Study of multiplex real - time fluorescent PCR detection method for animal originated components in cosmetics
DNA of animal originated components in paste,liquid and solid form cosmetic products were sampled using improved Chelex - 100 combined with Glass Milk method. According to the donkey,horse and cattle mitochondrial 16S rRNA gene sequences,the universal primers and specific probes were designed. Then a multiplex real - time fluorescent PCR assay system to detect the species of animal originated (donkey,horse and cattle)components in cosmetic products was established. All the detection limit of all cosmetics achieves 0. 001 ng. Samples from marketed cosmetics such as creams,lotions and facial masks were detected by this way. The reliability and accuracy of this multiplex real - time fluorescent PCR assay were confirmed.
cosmeticsmultiplex real - time fluorescent PCRanimal originated components
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