Determination of atropine in cream cosmetics by ultra performance liquid chromatography-quadrupole/time-of-flight high-resolution mass spectrometry
A quick method for detecting atropine in cream cosmetics was devised using ultra performance liquid chromatography-quadrupole/time-of-flight high-resolution mass spectrometry(UPLC-QTOF-MS/MS),and a different approach was created for establishing an atropine database.The samples were ultrasonically extracted with 80％(V/V)acetonitrile solution.After centrifugation,the extracts were separated and filtered.The separation was performed on an ACQUITY UPLC® BEH C18(2.1 mm×100 mm,1.7 μm)column in electrospray positive ionization mode with MSE mode for characterization,multiple reaction monitoring(MRM)mode for data acquisition and quantification by external standard method of matrix solvent standard solution.The atropine shows a good linearity(R2>0.9990)in the range of 3.0-100.0 ng/mL.The limit of detection(LOD)of the method is 1.0 ng/mL and the limit of quantification(LOQ)is 3.0 ng/mL.The average recoveries of atropine are 107.9％,97.0％and 105.3％at the spiked levels of 20,50 and 80 ng/mL,respectively.The RSDs are 4.2％,3.0％and 3.2％,respectively(n=6).This method is simple,sensitive and suitable for the rapid screening and quantitative analysis of atropine in cosmetics.
ultra performance liquid chromatography-quadrupole/time-of-flight high-resolution mass spectrometry(UPLC-QTOF-MS/MS)alkaloidsatropinecream cosmetic
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