Determination of four polyphenols and study on antioxidant and anti-inflammatory activities of extracts from callus of Eryngium maritimum L.
The preparation of extracts from callus of Eryngium maritimum was conducted by hot water reflux method.A method for simultaneous determination of rosmarinic acid,cryptochlorogenic acid,hyperoside,quercetin-3-O-(6″-O-acetyl)-β-D-glucopyranoside in the callus extract of Eryngium maritimum was established based on high performance liquid chromatography.The scavenging ability of the extracts on DPPH and ABTS free radicals were determined.The screening samples were also assayed by the CCK-8 method to determine the concentration of drug administration and a model of HaCaT cell inflammation induced by Cutibacterium acnes was established to evaluate its anti-inflammatory efficacy.The results show that the extracts contain the highest amount of cryptochlorogenic acid at 5.23 mg/g,and the contents of rosmarinic acid,quercetin-3-O-(6″-O-acetyl)-β-D-glucopyranoside and hyperin were 1.30,0.50 and 0.08 mg/g,respectively.The extracts display antioxidant and anti-inflammatory abilities.The IC50 values of the extracts on DPPH and ABTS free radical scavenging ability are 0.323 9 and 0.296 5 mg/mL,respectively,and also have significant inhibitory effects on the secretion of IL-1β and IL-8 in HaCaT cells induced by Cutibacterium acnes(P<0.05).
国家科技期刊平台
NSTL
万方数据
维普
