Determination of functional components of Glycyrrhizae,Ginseng and Scutellariae in facial masks by UHPLC-MS/MS
An analytical method was established for the determination of Glycyrrhizae functional components(liquiritin and glycyrrhizic acid),Ginseng functional components(ginsenoside Rg1,ginsenoside Rb1,ginsenoside Re)and Scutellariae functional components(baicalin)in facial mask cosmetics with ultra-high performance liquid chromatography triple quadrupole mass spectrometry(UHPLC-MS/MS).This method was used to monitor the risk of 20 batches of samples sold on the market.The samples were extracted by ultrasonic method using 0.1%formic acid in methanol.The mobile phase was 0.1%formic acid aqueous solution-acetonitrile solution,and the gradient elution was carried out.The samples were separated on the ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8 μm)column and detected by UHPLC-MS/MS negative ion mode.At the same time,the pretreatment conditions and UHPLC-MS/MS conditions were optimized and the method was verified.The results show that the method shows good linearity in the mass concentration range of 0.25-100 ng/mL for liquiritin and baicalin,and 0.50-200 ng/mL for ginsenoside Rg1,ginsenoside Rb1,ginsenoside Re and glycyrrhizic acid,with R no less than 0.998.The limits of detection are in the range of 0.41-6.4 μg/kg(S/N=3).The average recoveries in the sample matrix of facial masks are from 95.9%to 109.1%,with the relative standard deviations(RSDs)less than 4.9%(n=6).This method is suitable for the determination of ginseng functional components in the ginseng cosmetics.
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万方数据
维普
