Simultaneous determination of 24 coumarin compounds in cosmetics by high performance liquid chromatography and verification by liquid chromatography-tandem mass spectrometry
An analytical method for simultaneous determination of 24 coumarin compounds in cosmetics was established by high performance liquid chromatography.Samples with certain fluidity,such as lotions,creams,and water-based samples,were directly extracted with methanol by ultrasonic treatment,while solid samples(lipsticks,pressed powders,etc.)were ultrasonically extracted with mixed solvent of tetrahydrofuran-methanol(1∶1).The compounds were separated on a CAPCELL CORE MGII C18(4.6×250 mm,5 μm)column,and gradient elution was carried out with methanol and 0.02 mol/L sodium dihydrogen phosphate buffer(pH 3.5)as the mobile phase.The flow rate was 1.0 mL/min.The quantification was done by external standard method,with PDA detector at multi-wavelength,and the suspected positive samples were confirmed by liquid chromatography-tandem mass spectrometry.The results showed that the 24 coumarin compounds showed good linearity,with correlation coefficient≥0.999 6 in their respective mass concentration range.The limits of detection and limits of quantification were 2-19 mg/kg and 5-63 mg/kg,respectively.At the three spiked levels of low,medium and high,the average recoveries ranged from 94.4%to 106.4%,and the relative standard deviations were between 0.5%and 3.3%.This method was simple,accurate and suitable for the determination of 24 coumarin compounds in cosmetics.
coumarincosmetichigh performance liquid chromatographyliquid chromatography-tandem mass spectrometry
国家科技期刊平台
NSTL
万方数据
维普
