Determination of tolnaftate and liranaftate in cosmetics by high performance liquid chromatography and verification by high performance liquid chromatography-tandem mass spectrometry
A method for the determination of tolnaftate and liranaftate in cosmetics by high performance liquid chromatography(HPLC)and verification by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)was established.Samples(including 5 kinds of matrix:cream,emulsion,liquid,gel,and oil)were extracted with acetonitrile,mixed thoroughly by vortex and ultra-sonication.In HPLC method,the separation was performed by C18 column of 5 μm particle sizes using acetonitrile and 0.1％formic acid water as mobile phases at the wavelength of 258 nm and 283 nm.The HPLC-MS/MS method employed a rapid resolution C18 column of 1.8 μm particle sizes and the same mobile phases as the HPLC method.The target compounds were analyzed by multiple reaction monitoring(MRM)in electro-spray ionization(ESI)mode.Both the HPLC and HPLC-MS/MS methods show good performance in methodology validation.In HPLC method,the linearities of tolnaftate and liranaftate are in the range of 0.1-10.0 mg/L and 0.2-20.0 mg/L,respectively(correlation coefficient,R2>0.99),the limits of detection(LOD)are 3.0 mg/kg and 6.0 mg/kg,the limits of quantitation(LOQ)are 10 mg/kg and 20 mg/kg,and the spiked recoveries are between 93.3％and 107.9％(relative standard deviation,RSD<5％).While in HPLC-MS/MS method,the linearities of tolnaftate and liranaftate both are 0.05-5.00 μg/L(R2>0.99),the LODs are 0.002 mg/kg,the LOQs are 0.005 mg/kg,and the spiked recoveries are between 84.0％and 112.8％(RSD<5％).The method established in this study is simple,rapid,with strong specificity and high accuracy and is suitable for the determination of tolnaftate and liranaftate in cosmetics.
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