Study on the anti-aging and antioxidant effects of isosinensetin
The study aimed to investigate the anti-aging and antioxidant effects of isosinensetin(ISO)on skin aging mice induced by D-galactose(D-gal).The mice were divided into 6 groups(n=12):C group,D-gal group,D-gal+L-ISO group,D-gal+M-ISO group,D-gal+H-ISO group and D-gal+H-ISO+ML385 group.The skin aging mouse model was established by subcutaneous injection of D-gal of 1 g/(kg·d)into the back of mice for 6 weeks.From the day of D-gal injection,mice in C group and D-gal group were applied with snow cream on their back depilation area,while mice in D-gal+L-ISO group,D-gal+M-ISO group and D-gal+H-ISO group were applied with 50,100 and 200 mg/kg isosinensetin cream,respectively.The mice in D-gal+H-ISO+ML385 group were applied with 200 mg/kg isosinensetin cream and intraperitoneally injected with 30 mg/(kg·d)nuclear factor E2 related factor 2(Nrf2)inhibitor ML385.The application amount was 2 mg/cm2.After 6 weeks of transdermal administration,the body weight,skin water content,skin antioxidant index,hydroxyproline(HYP)and hyaluronic acid(HA)levels were measured.The skin morphology was observed by HE staining.The transcriptional levels of COL1A1,COL3A1,matrix metalloproteinase(MMP)-1 and MMP-3 in skin were detected by RT-qPCR.The protein levels of Nrf2(nucleus),Kelch-like ECH associated Protein 1(Keap1)and heme oxygenase-1(HO-1)in skin were detected by Western blotting.The results show that all three doses of isosinensetin increases skin water content,improves skin morphology,increases superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)levels,decreases malondialdehyde(MDA)levels,increases HYP and HA levels,increases COL1A1 and COL3A1 mRNA levels,decreases MMP-1 and MMP-3 mRNA levels,increases Nrf2(nucleus)and HO-1 protein levels,and decreases Keap1 protein levels in skin aging mice(P＜0.05).ML385 attenuates the anti-aging and antioxidant effects of isosinensetin(P＜0.05).In short,transdermal administration of isosinensetin can improve the anti-aging and antioxidant ability of D-gal-induced skin aging mice by activating Nrf2 pathway.
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