Determination of 36 stimulants in cosmetics by ultra performance liquid chromatography-tandem mass spectrometry
An ultra performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS)method was developed for the simultaneous determination of 36 stimulants including anabolic agents,β2 agonists,glucocorticoids,irritants,and cannabinoids in cosmetics.In this study,pretreatment operation and instruments parameters were also optimized.Cosmetic samples were ultrasonically extracted by methanol,and then the separation of 36 stimulants was carried out on a Waters ACQUITY UPLC BEH C18 column(3.0 mm×150 mm,1.7 μm)by gradient elution,using 0.1%acetic acid solution and methanol-acetonitrile(1∶9,by volume)as the mobile phase.The 36 target compounds were analyzed in positive and negative electrospray ionization mode under multiple reaction monitoring(MRM)mode.Meanwhile,the analytes were quantified by external standard method,and the linear regression analysis data show that there are good linear relationships for 36 target compounds in the mass concentration range of 1.0-20 ng/mL,with their correlation coefficients(r)greater than 0.995.The limits of detection(LOD)and limits of quantitation(LOQ)of the method are in the ranges of 1.0-3.0 μg/kg and 3.3-9.9 μg/kg,respectively.The recoveries for 36 target compounds in three kinds of matrix samples at three spiked levels are between 80.6%and 115.3%,with the relative standard deviations(RSD)of 0.5%-12.9%(n=5).The method is simple,rapid,high sensitivity,accurate and suitable for the rapid detection of many stimulants in cosmetics.
ultra performance liquid chromatography-tandem mass spectrometrystimulantcosmetics
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