Qualitative screening and quantitative analysis of 42 restricted hair dyes in oxidative hair color products by high performance liquid chromatography
A method for qualitative screening and quantitative analysis of 42 restricted hair dyes in oxidative hair color products by high performance liquid chromatography(HPLC)was established.The sample was ultrasonically extracted in mixed solvent of aqueous sodium bisulfite solution and ethanol.Firstly,the liquid chromatography condition for qualitative screening of 42 dyes and for quantitative analysis of 35 of them was established,which was as follows:The mobile phase was the mixed solvent of aqueous solution of 0.02 mol/L ammonium acetate and acetonitrile;gradient elution was carried out on a TechMate AQUA-STIII column;the detection wavelengths were 235 nm and 280 nm.If the other seven dyes including 2,6-dimethoxy-3,5-pyridinediamine·HCl,2-methyl-5-hydroxyethylaminophenol,3-nitro-p-hydroxyethylaminophenol,pyrazolone methyl pyrazolone,hydroxypropyl bis(N-hydroxyethyl-p-phenylenediamine)·HCl,4-chlororesorcinol and 6-hydroxyindole were detected in the products,another liquid chromatography condition was required for their quantitative analysis,which was as follows:The mobile phase was the mixed solvent of aqueous solution of 0.02 mol/L ammonium acetate and acetonitrile;isocratic elution was carried out on a Waters Symmetry C18 column;the detection wavelength was 235 nm.The results showed that the limits of detection(LODs)of the 42 dyes were from 10µg/g to 101µg/g,while the limits of quantification(LOQs)were from 33µg/g to 337µg/g.The 42 dyes all had good linearities in their respective range,with correlation coefficients(r)higher than 0.9998.The average spiked recoveries of 42 dyes at four levels were from 81.3%to 119.6%,with RSDs no more than 5%.
high performance liquid chromatography(HPLC)oxidative hair color productrestricted hair dyecosmetic
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