Rapid determination of free ethanolamine compounds in cosmetics by liquid chromatography-tandem mass spectrometry
A stable isotope dilution-liquid chromatography-tandem mass spectrometry method was established for the determination of three free ethanolamine compounds in cosmetics.The ethanolamine compounds were extracted with isopropanol and separated by Kinetex HILIC chromatographic column with isocratic elution using acetonitrile and 0.1%formic acid-water solution(V/V).The samples were ionized by positive ion mode of electrospray ionization source and determined by multiple reaction monitoring(MRM)mode.Optimization was conducted using single factor experiments for the best conditions of determination.The optimum determination conditions are using isopropanol by ultrasonic extraction for 20 min.Under this combination of conditions,the ranges of internal standard curves for monoethanolamine,diethanolamine,and triethanolamine are 5.0-200 μg/L,2.0-200 μg/L and 1.0-200 μg/L,respectively,with the correlation coefficient R2 greater than 0.999.The average recovery rate ranges from 87.1%to 97.0%,and relative standard deviations(RSDs)are between 1.68%-6.57%.The limit of detection and limit of quantification are 3.00-15.0µg/kg and 10.0-50.0µg/kg,respectively.The established method is used for the detection of 61 cosmetic samples.The method is simple operation,accurate quantification,stable recovery,high sensitivity and high precision and suitable for the determination of free ethanolamine compounds in cosmetics.
liquid chromatography-tandem mass spectrometryrapid determinationcosmeticsethanolamine compounds
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