首页|全氟辛酸对成骨细胞骨形成和破骨细胞骨吸收作用研究

全氟辛酸对成骨细胞骨形成和破骨细胞骨吸收作用研究

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  • 国家科技期刊平台
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考察全氟辛酸(PFOA)对成骨细胞骨形成和破骨细胞骨吸收的作用和机制,为探索PFOA暴露引起骨丢失提供科学依据.采用原代新生大鼠颅盖骨分离成骨细胞,考察1,10和100 μmol/L PFOA对成骨细胞增殖,碱性磷酸酶(ALP)活性和骨结节形成的影响;采用巨噬细胞集落刺激因子(M-CSF)及核因子κB受体激活因子配体(RANKL)诱导大鼠骨髓单核细胞分化破骨细胞模型,考察1,10和100 μmol/L PFOA对破骨细胞活力,抗酒石酸酸性磷酸酶(TRAP)活性和骨吸收陷窝形成指标的影响;采用Western blot考察成骨细胞护骨素(OPG)和RANKL蛋白表达.PFOA在1和10μmol/L时促进成骨细胞活力,但对ALP活性和骨结节形成无影响.100 μmol/L PFOA可显著抑制细胞增殖,ALP活性和骨结节形成,同时抑制OPG/RANKL蛋白表达的比例.PFOA对破骨细胞活力无影响,10和100μmol/L PFOA可显著抑制促进TRAP活性和骨吸收陷窝形成面积.PFOA抑制成骨细胞骨形成作用可能通过下调Wnt通路,通过抑制OPG/RANKL/RANK通路,促进破骨细胞骨吸收作用.
Effects of PFOA on osteoblastic bone formation and osteoclastic bone resorption
Perfluorooctanoic acid(PFOA)exposure level in human is negatively correlated with bone density according to the epidemiological research.Our study is aim to investigate the effects and mechanisms of PFOA on osteoblastic bone formation and osteoclastic bone resorption.Primary osteoblasts were isolated from the calvaria of newborn rats,and the cell proliferation,alkaline phosphatase(ALP)activity,and bone nodule formation were detected after 1,10,and 100 μmol/L PFOA treateatment for 24 h.The osteoclasts model were differentiation from bone marrow monocyte,which induced by Macrophage colony-stimulating factor(M-CSF)and Nuclear factorκB receptor-activating factor ligand(RANKL),and the effects on cell viability,anti-tartaric acid phosphatase(TRAP)activity,and bone resorption pit formation were investigated.The expression of OPG and RANKL proteins in osteoblasts were conducted by Western blot.The results show that PFOA at the concentration of 1 μmol/L and 10 μmol/L can promote osteoblast proliferation,but has no effect on ALP activity and bone nodule formation.100 μmol/L PFOA can significantly inhibit cell proliferation,ALP activity,and bone nodule formation,as well as the proportion of OPG/RANKL protein expression.PFOA has no effect on osteoclast viability,and 10 μmol/L and 100 μmol/L PFOA can significantly inhibit the TRAP activity and the area of bone resorption pits.The conclusion can be draw that high concentration of PFOA can inhibit the bone formation of osteoblasts by suppressing WNT pathway and promote osteoclast bone resorption by inhibiting the OPG/RANKL/RANK pathway.

perfluorooctanoic acidbone lossosteoblastosteoclastOPG/RANKL/RANK pathway

薛黎明、林元杰、金玉娥、徐佳乐、卢大胜、汪国权

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上海市疾病预防控制中心 国家环境保护新型污染物环境健康影响评价重点实验室,上海 200336

全氟辛酸 骨丢失 成骨细胞 破骨细胞 OPG/RANKL/RANK通路

上海市自然科学基金面上项目上海市自然科学基金面上项目上海市"医苑新星"青年医学人才-公共卫生领导者项目上海市卫生健康委面上项目上海市社会发展科技攻关计划上海市加强公共卫生体系建设三年行动计划重点学科建设项目(2023-2025)

21ZR145460022ZR1453400沪卫人事[2021]99号20204020821DZ1202100GWVI-11.1-41

2024

10.3969/j.issn.2097-2806.2024.06.004

日用化学工业(中英文)
中国日用化学工业研究院

日用化学工业(中英文)

CSTPCD北大核心
影响因子：0.553
ISSN：1001-1803
年,卷(期)：2024.54(6)
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