Determination of 5 whitening agents in cosmetics using UPLC-MS/MS
A method was established for quantitative determination of five whitening agents in cosmetics using ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The samples were extracted with methanol-water solution(70∶30),ultrasonic extraction for 30 minutes and then separated by Agilent column(ZORBAX RR StableBond Aq)followed by gradient elution.An electrospray ionization(ESI)source in negative ion mode was utilized for multiple reaction monitoring(MRM)scanning.The results show that under optimal conditions no obvious matrix effect exists in 3 different cosmetic matrices.Among the three cosmetics with different types of matrices,good linearity is obtained for five whitening agents in the range of 1.0-200.0 ng/mL,and the correlation coefficients(R2)are more than 0.996.The limit of detection(LOD)and limit of quantitation(LOQ)are 0.01-0.1 mg/kg and 0.03-0.3 mg/kg,respectively.Spike recovery is performed on three cosmetics at three different spike levels(1,2,and 10 times of the LOQ),and the average recovery range is 82.6%-104.8%with a relative standard deviation of 2.1%-8.6%(n=6).This method is easy to implement,efficient and accurate.It is suitable for quantitative analysis of five whitening agents,including deoxyarbutin,α-arbutin,β-arbutin,hydroquinone,and ursolic acid in common cosmetics,providing technical support for the regulation and risk monitoring of the cosmetics industry.
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