首页|超声辅助酶法降解枳实果胶及其衍生物抗氧化性能

超声辅助酶法降解枳实果胶及其衍生物抗氧化性能

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采用超声辅助酶法对枳实果胶进行降解,获得枳实果胶降解衍生物.以抗氧化活性为指标,考察了超声预处理时间、酶种类、酶添加量等因素对枳实果胶降解衍生物抗氧化活性的影响.利用滴定法、凝胶色谱法和红外光谱等技术分析了枳实果胶降解衍生物中半乳糖醛酸含量、分子量分布以及结构特性,以验证果胶降解衍生物抗氧化构效关系.结果表明:当果胶酶作为降解酶,酶用量为6 000 U/g,超声预处理时间为20 min时,制备的枳实果胶降解衍生物的抗氧化活性最佳,此条件下其质量浓度为3 mg/mL时,对DPPH自由基的清除率为62.94%,对ABTS自由基的清除率为52.67%,还原能力为0.962 7,与未降解的枳实果胶相比,抗氧化活性显著提高.枳实果胶降解衍生物中半乳糖醛酸含量随降解工艺的不同存在明显差异,且半乳糖醛酸含量与抗氧化活性呈正相关.此外,枳实果胶降解衍生物分子量更低,且甲氧基含量也明显降低,但降解过程并没有改变果胶的吡喃糖结构.研究结果为枳实果胶的高值化利用提供理论依据和参考.
Ultrasound-assisted enzymatic degradation to obtain pectin derivatives from Citrus aurantium L.and its antioxidant properties
In this paper,pectin derivatives obtained from the degradation of Citrus aurantium L.pectin was prepared by ultrasound-assisted enzymatic method.The effects of ultrasound pretreatment time,enzyme type and amount of enzyme addition on the antioxidant activity of degradation derivatives of Citrus aurantium L.pectin were investigated.Then the content,molecular weight distribution and structural properties of galacturonic acid from the degradation derivatives of Citrus aurantium L.pectin were analyzed by titration gel chromatography and infrared spectroscopy to verify the plausible explanation for the enhanced antioxidant activity of pectin degradation derivatives.The results confirm that the best scavenging rate of DPPH radicals and ABTS radicals as well as the reducing ability of potassium ferricyanide of the pectin degradation derivatives are obtained,when pectinase is selected as the degrading enzyme with the dosage of 6 000 U/g and the ultrasound-pretreatment time is 20 min.Under these conditions,the degradation derivatives of Citrus aurantium L.pectin prepared at a mass concentration of 3 mg/mL,shows a more significant increasing in antioxidant activity with a 62.94%scavenging of DPPH radicals,a 52.67%scavenging of ABTS radicals and a reducing capacity of 0.962 7,compared to the undegraded pectin.The content of galacturonic acid in the degradation derivatives of Citrus aurantium L.pectin varies significantly with the degradation process.Also it is verified that it is a positive correlation between galacturonic acid content and antioxidant activity,and that a lower molecular weight and lower methoxy content of the degradation derivatives are obtained compared to Citrus aurantium L.pectin,but the pyranose structure of the pectin is not changed in the degradation process.The results of this study provide theoretical basis and reference for the high-value utilization of Citrus aurantium L.pectin.

Citrus aurantium L.pectinultrasound assisted enzyme methoddegradation derivativesantioxidant activityconstitutive analysis

钱伟、阳斌、邵博、万力、周军

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广东环境保护工程职业学院 环境监测学院,广东 佛山 528216

中南林业科技大学 材料科学与工程学院,湖南 长沙 410004

枳实果胶 超声辅助酶法 降解衍生物 抗氧化活性 构效分析

2023年度广东省教育科学规划课题高等教育专项广东省高校特色创新项目广东省高职院校高水平专业群广东环境保护工程职业学院2020年度院长基金项目(科学研究类)

2023GXJK7942021KTSCX250GSPZYQ2020004K620521042304

2024

10.3969/j.issn.2097-2806.2024.08.009

日用化学工业(中英文)
中国日用化学工业研究院

日用化学工业(中英文)

CSTPCD北大核心
影响因子：0.553
ISSN：1001-1803
年,卷(期)：2024.54(8)