Simultaneous determination of 27 preservatives in toothpaste by high-performance liquid chromatography
A method of high-performance liquid chromatography(HPLC)was established for simultaneous determination of 27 preservatives in toothpaste.Toothpaste samples were extracted with 50％aqueous methanol solution,ultrasonicated and then filtered.The target compounds were separated on a C18 column(250 mm × 4.6 μm,5 μm)with the mobile phase consisting of acetonitrile and aqueous phosphoric acid solution,the column temperature was 30 ℃,the sample volume was 10 μL,the flow rate was 1 mL/min,and diode array detector was used.Under the optimal experimental conditions,good linear relationship was obtained and the correlation coefficient(R2)was greater than 0.999 5.This method was accurate and sensitive,which was suitable for the qualitative and quantitative analysis of 27 preservatives in toothpaste.
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