A new method for the simultaneous and rapid determination of 14 thiazides in cosmetics by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established.The cosmetic samples were dispersed with methanol and ultrasonic extraction,and the filtrate was analyzed by a Agilent RRHD Eclipse Plus Zorbax C18 chromatographic column(3.0 mm×100 mm,1.8 μm),with 0.2%formic acid aqueous solution and methanol as mobile phases under gradient elution.The condition of mass spectrometry was negative ions scanning with multiple reaction monitor mode(MRM)under the electrospray ionization source.For the representative matrix samples(including water aqua,gel,lotion,cream,powder and essential oil),thiazides tested show the correlation coefficients for linear calibration curves are in the range of 0.990 7-0.9996 in the corresponding concentration range.The limits of detections(LODs)of 14 thiazides are in the range of 0.004 0-0.100 0 μg/g,and the limits of quantitations(LOQs)are in the range of 0.008 0-0.400 0 μg/g.The recoveries of low,medium and high concentrations ranged 85.9%-114.4%,with the relative standard deviations(n=6)ranged 0.8%-11.8%.The established method is simple,effective,high sensitive,good repeatability,qualitatively reliable,quantitatively accurate,and is suitable for the determination of 14 thiazides in different matrixes of cosmetics.
维普
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