Determination of 21 Veterinary Drugs in Cow and Goat Milk and Milk Powder by QuEChERS Combined with Ultra Performance Liquid Chromatography-Quadrupole/Orbitrap Mass Spectrometry
Determination of 21 Veterinary Drugs in Cow and Goat Milk and Milk Powder by QuEChERS Combined with Ultra Performance Liquid Chromatography-Quadrupole/Orbitrap Mass Spectrometry
A method for the determination of 21 veterinary drugs in cow and goat milk and milk powder was established by the QuEChERS(quick,easy,cheap,effective,rugged,safe)sample pretreatment method combined with ultra performance liquid chromatography-quadrupole/orbitrap mass spectrometry(UPLC-Q/Orbitrap MS).Chromatographic conditions,mass spectrometric parameters and pretreatment conditions were optimized.Samples were extracted with acetonitrile in the presence of anhydrous sodium sulfate,purified using a mixture of primary secondary amine(PSA)and C18,separated on an Accucore RP-MS column(100 mm × 2.1 mm,2.7 μm),and detected by UPLC-Q/Orbitrap MS.Good linearity was observed for all the analytes in the investigated concentration ranges with correlation coefficients(r2)of greater than 0.99.The limit of quantification(LOQ)of the proposed method was 0.5 μg/kg.The average recoveries at spiked concentration levels of 0.5,2.0 and 10.0 μg/kg were 67.2％-110.2％with relative standard deviations(RSDs)of 3.6％-17.4％(n=6).The method is simple and sensitive,and suitable for simultaneous detection of the 21 veterinary drugs in cow and goat milk and milk powder.
关键词
QuEChERS/静电场轨道阱质谱/抗虫药/消炎药/糖皮质激素
Key words
QuEChERS/electrostatic field orbitrap mass spectrometry/antiparasitics/antiphlogistics/glucocorticoids
维普
万方数据