首页|基于实时荧光定量环介导等温扩增技术快速检测发酵乳中嗜热链球菌和保加利亚乳杆菌

基于实时荧光定量环介导等温扩增技术快速检测发酵乳中嗜热链球菌和保加利亚乳杆菌

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
基于实时荧光定量环介导等温扩增(quantitative loop-mediated isothermal amplification,qLAMP)技术建立发酵乳中嗜热链球菌和保加利亚乳杆菌活菌数快速定量方法.通过优化嗜热链球菌和保加利亚乳杆菌叠氮溴化丙锭(propidium monoazide,PMA)处理条件,对菌浓度对数与相应的循环阈值进行线性拟合,构建标准曲线,建立PMA-qLAMP检测方法.结果表明,该方法对嗜热链球菌的最低定量限为7.2×103 CFU/g,对保加利亚乳杆菌的最低定量限为5.6×104 CFU/g,将该方法应用于市售发酵乳产品活菌数定量检测,显示出良好的灵敏度和实用性.
Development of Quantitative Loop-Mediated Isothermal Amplification for Rapid Detection of Streptococcus thermophilus and Lactobacillus bulgaricus in Fermented Milk
In this study,a rapid and quantitative method for the detection of viable bacterial counts of Streptococcus thermophilus and Lactobacillus bulgaricus in fermented milk was established using propidium monoazide(PMA)combined with quantitative loop-mediated isothermal amplification(PMA-qLAMP).The PMA treatment conditions for S.thermophilus and L.bulgaricus were optimized.A linear relationship was fitted between the logarithm of bacterial concentration and cycle threshold(Ct)value.The limit of quantification for S.thermophilus and L.bulgaricus was 7.2×103 and 5.6×104 CFU/g,respectively.The PMA-qLAMP method was applied to measure viable bacterial counts in commercial fermented milk,demonstrating good sensitivity and practicality.

fermented milkloop-mediated isothermal amplificationpropidium monoazidelactic acid bacteria

周帅康、胡连霞、王孟阳、李苗、卢涵、王世杰

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河北科技大学食品与生物学院,河北 石家庄 050091

石家庄学院化工学院,河北 石家庄 050035

沧州市食品药品检验所,河北 沧州 060012

君乐宝乳业集团股份有限公司,河北 石家庄 050221

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发酵乳 环介导等温扩增 叠氮溴化丙锭 乳酸菌

中央引导地方科技发展资金项目(科技创新基地项目)河北省"三三三人才工程"重点项目河北省创新能力提升计划项目石家庄市产业技术研究院建设项目

236Z2804GA2023100222567685H228790859A

2024

10.7506/rykxyjs1671-5187-20240505-033

乳业科学与技术
上海奶业行业协会　光明乳业股分有限公司技术中心　上海市奶牛研究所　上海乳品培训研究中心

乳业科学与技术

CSTPCD
影响因子：0.619
ISSN：1671-5187
年,卷(期)：2024.47(3)