液相色谱法测定特殊医学用途婴幼儿配方食品中VC
Determination of Vitamin C in Formulas for Special Medical Purpose Intended for Infants by Liquid Chromatography
吴秀萍 1程白羽 1许小茜 1姜睿彧1
作者信息
- 1. 上海市质量监督检验技术研究院,上海 200233
- 折叠
摘要
建立液相色谱法检测特殊医学用途婴幼儿配方食品中VC含量的分析方法.样品还原处理后,经HLB固相萃取柱净化、微孔滤膜过滤后进行亲水作用液相色谱法测定,外标法定量,对检测方法进行验证.结果表明:在质量浓度为0～50 µg/mL范围内其线性相关系数≥0.999,定量限为3.0 mg/100 g,加标回收率为95.2%～102.8%,相对标准偏差为1.22%～3.62%;该方法具有操作简单、结果准确和重复性好等特点,适用于特殊医学用途婴幼儿配方食品中VC含量的测定.
Abstract
A liquid chromatography(LC)method for the determination of vitamin C in formulas for special medical purpose intended for infants was established and validated.The sample was reduced,purified using an HLB solid phase extraction(SPE)cartridge,filtered through a 0.45 μm filter,and analyzed by hydrophilic interaction liquid chromatography(HILIC).The calibration curve was found to be linear in the concentration range of 0-50 μg/mL with correlation coefficient over 0.999.The limit of quantitation(LOQ)was 3.0 mg/100 g.Recoveries of spiked samples ranged from 95.2%to 102.8%with relative standard deviation(RSD)between 1.22%and 3.62%.The method is characterized by simple operation,accurate results and good reproducibility and suitable to determine vitamin C in formulas for special medical purpose intended for infants.
关键词
特殊医学用途婴幼儿配方食品/VC/液相色谱法/亲水作用液相色谱Key words
formulas for special medical purpose intended for infants/vitamin C/liquid chromatography/hydrophilic interaction liquid chromatography引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据