Membrane Separation Followed by Ion Exchange Chromatography for Preparation of High-Purity α-Lactalbumin
In this study,ion exchange chromatography was employed to prepare high-purity α-lactalbumin from whey protein powder obtained from raw cow's milk by sequential membrane separation and spray drying.The results showed that α-lactalbumin constituted 21.04%of the true protein in the permeate obtained using a 50-nm pore-sized ceramic membrane with three-fold concentration,one cycle of microfiltration and two cycles of washing,which was higher than that obtained using a 100-nm pore-sized ceramic membrane(15.84%).The permeate was spray dried and separated by ion exchange chromatography.Good chromatographic separation of α-lactalbumin and β-lactoglobulin was achieved with increasing NaCl concentration from 0 to 0.5 mol/L at up to 10 column volumes,and 98.18%pure α-lactalbumin and 97.82%pure β-lactoglobulin were obtained.The results of this study provide support for the industrial preparation of high-purity α-lactalbumin.
α-lactalbuminβ-lactoglobulinseparation and purificationmembrane separationion exchange chromatography
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