首页|胀包学生饮用乳中不解糖嗜胨菌的鉴定

胀包学生饮用乳中不解糖嗜胨菌的鉴定

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以学生饮用乳(发酵乳)来源的不解糖嗜胨菌为研究对象,基于分离计数、形态学观察、生化鉴定、基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time-of-flight mass spectrometry,MALDI-TOF MS)和全基因组测序技术,建立适用于不解糖嗜胨菌的鉴定方法.结果表明:不解糖嗜胨菌在PCA培养基上厌氧环境下生长较适;在全自动微生物鉴定系统上鉴定为不解糖嗜胨菌,置信度98%;MALDI-TOF MS鉴定为不解糖嗜胨菌,鉴定比对值为2.241.通过全基因组测序,平均核苷酸一致性分析结果为98.58%.利用本研究建立的方法能够满足不解糖嗜胨菌的快速、准确鉴定,符合食品安全检测需求.
Identification of Peptoniphilus asaccharolyticus Isolated from Spoiled Fermented Milk Intended for Students
This study focuses on the identification of Peptoniphilus asaccharolyticus from spoiled fermented milk intended for students based on isolation,counting,morphological observation,biochemical identification,matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS),and whole-genome sequencing.The results showed that the isolate grew well on PCA medium under anaerobic conditions and was identified as P.asaccharolyticus on a fully automated microbial identification system with a confidence level of 98%.By MALDI-TOF MS,it was confirmed as P.sasaccharolyticus with a comparison value of 2.241.The result of whole genome sequencing showed an average nucleotide identity(ANI)of 98.58%.The proposed method allows rapid and accurate identification of P.sasaccharolyticus and can meet the requirements of food safety detection.

fermented milk intended for studentsPeptoniphilus asaccharolyticusstrain identificationmatrix-assisted laser desorption ionization time-of-flight mass spectrometrywhole genome sequencing

高佳丽、王晓茹、肖霄、陈佳、孟德尚、孙伟

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石家庄学院化工学院,河北 石家庄 050227

北京市茶叶质量监督检验站,北京 100000

河北省知识产权保护中心,河北 石家庄 050000

学生饮用乳(发酵乳) 不解糖嗜胨菌 菌株鉴定 基质辅助激光解吸电离飞行时间质谱 全基因组测序

河北省高等学校科学技术研究项目

ZC2023112

2024

10.7506/rykxyjs1671-5187-20240708-055

乳业科学与技术
上海奶业行业协会　光明乳业股分有限公司技术中心　上海市奶牛研究所　上海乳品培训研究中心

乳业科学与技术

CSTPCD
影响因子：0.619
ISSN：1671-5187
年,卷(期)：2024.47(5)