Identification of Peptoniphilus asaccharolyticus Isolated from Spoiled Fermented Milk Intended for Students
高佳丽 1王晓茹 1肖霄 1陈佳 1孟德尚 2孙伟3
扫码查看
点击上方二维码区域，可以放大扫码查看
作者信息
1. 石家庄学院化工学院,河北 石家庄 050227
2. 北京市茶叶质量监督检验站,北京 100000
3. 河北省知识产权保护中心,河北 石家庄 050000
折叠
摘要
以学生饮用乳(发酵乳)来源的不解糖嗜胨菌为研究对象,基于分离计数、形态学观察、生化鉴定、基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time-of-flight mass spectrometry,MALDI-TOF MS)和全基因组测序技术,建立适用于不解糖嗜胨菌的鉴定方法.结果表明:不解糖嗜胨菌在PCA培养基上厌氧环境下生长较适;在全自动微生物鉴定系统上鉴定为不解糖嗜胨菌,置信度98%;MALDI-TOF MS鉴定为不解糖嗜胨菌,鉴定比对值为2.241.通过全基因组测序,平均核苷酸一致性分析结果为98.58%.利用本研究建立的方法能够满足不解糖嗜胨菌的快速、准确鉴定,符合食品安全检测需求.
Abstract
This study focuses on the identification of Peptoniphilus asaccharolyticus from spoiled fermented milk intended for students based on isolation,counting,morphological observation,biochemical identification,matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS),and whole-genome sequencing.The results showed that the isolate grew well on PCA medium under anaerobic conditions and was identified as P.asaccharolyticus on a fully automated microbial identification system with a confidence level of 98%.By MALDI-TOF MS,it was confirmed as P.sasaccharolyticus with a comparison value of 2.241.The result of whole genome sequencing showed an average nucleotide identity(ANI)of 98.58%.The proposed method allows rapid and accurate identification of P.sasaccharolyticus and can meet the requirements of food safety detection.
关键词
学生饮用乳(发酵乳)/不解糖嗜胨菌/菌株鉴定/基质辅助激光解吸电离飞行时间质谱/全基因组测序
Key words
fermented milk intended for students/Peptoniphilus asaccharolyticus/strain identification/matrix-assisted laser desorption ionization time-of-flight mass spectrometry/whole genome sequencing
维普
万方数据