Effects of high temperature and liquid nitrogen treatment on germination ability of equine prickly seeds
Horsethorn is a small perennial shrub of Magnolia legume,which has high feeding and ecological value,but the germination rate of seeds is low under natural conditions.The development of technical measures to improve the germination ability of horsethorn seeds will provide important support for the popularization and application of horsethorn.In this experiment,the horse thorn seeds harvested in 2022 were pretreated with high temperature(60℃),liquid nitrogen and their combination at different times,and the germination experi-ment was carried out.Four seed treatment times were designed for liquid nitrogen and high temperature respectively,and the treatment time for liquid nitrogen was 0.5min,1min,2min and 5min respectively.The treatment time at high temperature(60℃)was 10min,30min,60min and 180min respectively.Combined treatment includes high temperature treatment followed by liquid nitrogen treatment and liquid ni-trogen treatment followed by high temperature treatment.The experimental treatments included control(CK)under natural germination conditions,8 single treatments and 32 combined treatments,with a total of 41 treatments.The germination rate,germination potential,germination index and average germination time were calculated after the germination period,and the influence of different treatments on the germination ability ofhippophae spiny seeds was evaluated by membershiPfunction method.The germination rate was 55%under natural con-ditions,and there was no significant effect on the germination rate under different time of high temperature treatment(P>0.05).After 1min of liquid nitrogen treatment,the germination rate of hippophae sphinx seed was 78.5%,which was significantly higher than that of CK(P<0.05).In the combined treatment of high temperature followed by liquid nitrogen,the germination rate of 30min + liquid nitrogen for 5min and 60min + liquid nitrogen for 2min could reach 80%.In the combined treatment of liquid nitrogen followed by high temperature,the germination rate of liquid nitrogen for 1min + high temperature for 60min was the highest,reaching 82.5%,which was 27.5%higher than that of CK.Treatment with liquid nitrogen for 1min + high temperature for 60min can significantly improve the germination ability of horse thorn seeds,and this method is simple in operation,short in time and practical in production,so it is worth popularizing.
horsethornhigh temperature treatmentliquid nitrogen treatmentseed germination
万方数据
维普
