首页|耐热性紫花苜蓿新品种(系)筛选及其响应高温胁迫的生理机制研究

耐热性紫花苜蓿新品种(系)筛选及其响应高温胁迫的生理机制研究

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为筛选出适宜在南方推广种植的耐热性紫花苜蓿(Medicago sativa L.)新品种(系),本研究选取了 22 个不同秋眠级的紫花苜蓿品种(系)为试验材料,采用盆栽法探究热胁迫对其生长和生理特性的影响,基于隶属函数和聚类分析法进行耐热性综合评价.结果表明:随着胁迫温度升高,22 份紫花苜蓿的生理特性均受到不同程度的影响,结合聚类分析与隶属函数评价,所有材料被分为强耐热、中等耐热和热敏感3 个类群.其中,强耐热型包括南苜701、WL525HQ、赛迪 10,热敏感型包括南苜 801、赛迪7、维多利亚,其余材料为中等耐热型.从中挑出强耐热材料南苜 701、中等耐热材料吉利和热敏感材料南苜801 进行生理指标分析得出:热胁迫对强耐热材料南苜701 的影响最小,叶片中相对含水量下降幅度最小,电导率和丙二醛变化相对稳定,渗透调节物质如可溶性糖和脯氨酸大量积累,抗氧化酶如过氧化氢酶、过氧化物酶、超氧化物歧化酶和谷胱甘肽过氧化物酶等活性显著(P<0.05)高于其他材料,研究结果为适宜南方地区种植的紫花苜蓿新品种推广提供参考.
Comprehensive evaluation of high temperature and high heat resistance of 22 Medicago sativa L.varieties
The study aimed to screen out new varieties(lines)of heat-resistant alfalfa(Medicago sativa L.)that are suitable for cultivation in the southern regions,this study selected 22 different varieties(lines)of alfalfa with varying levels of dormancy as experimen-tal materials,and used pot cultivation method to explore the effects of heat stress on their growth and physiological characteristics.The heat tolerance of these varieties(lines)was comprehensively evaluated using membership function and clustering analysis.The results indicated that the physiological characteristics of 22 alfalfa varieties were impacted to varying extents by increasing stress temperatures.Through clus-ter analysis and membership function evaluation,all materials were categorized into three groups:strong heat resistance,moderate heat re-sistance,and thermal sensitivity.Among them,the strong heat resistant type includes Nanmu 701,WL525HQ,and Sardi 10,the ther-mal sensitive type includes Nanmu 801,Sardi 7,and Victoria,and the rest of the materials are medium heat resistant.Selecting the strong heat-resistant material Nanmu 701,the medium heat-resistant material Ghillie,and the heat sensitive material Nanmu 801 for physiolog-ical index analysis,it was found that heat stress had the smallest impact on the strong heat-resistant material Nanmu 701,the smallest de-crease in relative water content in leaves,stable changes in electrical conductivity and malondialdehyde,a large accumulation of osmoregu-latory substances such as soluble sugars content and proline.The activities of antioxidant enzymes such as catalase,superoxide dismutase,and glutathione peroxidase were significantly higher(P<0.05)than other materials.The research results provide reference for the promo-tion of new varieties of purple alfalfa suitable for planting in southern regions.

Medicago sativa L.heat stressheat resistance evaluationphysiological responses

张菲、李英主、李明峰、白史且、鄢家俊

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西南科技大学生命科学与工程学院,四川 绵阳 621010

四川省草原科学研究院,四川 红原 624400

紫花苜蓿 热胁迫 耐热性评价 生理响应

2024

草学
四川省草原科学研究院 四川省畜牧兽医学会

草学

影响因子:0.246
ISSN:2096-3971
年,卷(期):2024.(6)