Gene Sequences of CO Ⅰ and Cyt b of Ardea insignis and Molecular Phylogenetic Analysis of Genus Ardea
Ardea insignis is a rare species and listed as a critically endangered species in the IUCN red list of threatened species.It belongs to genus Ardea of family Ardeidae.There are very few studies carried on this species.We obtained CO Ⅰ and Cyt b gene sequences of A.insignis using PCR technology.The length of the Cyt b gene was 1 163 bp,and the A + T content (51.7％) was higher than that of C + G (48.3％).The length of the CO Ⅰ gene was 722 bp,and the A + T content (52.5％) was also higher than that of C + G (47.5％).The third site of both genes showed the most remarkable base bias.Combined with the sequences of the other 6 Ardea species from GenBank,the phylogenetic tree of genus Ardea was constructed.The result showed that 7 species of genus Ardea formed a monophyletic clade with the posterior support at 96％.A.alba and A.insignis were found at the basal position of this clade,indicating their early diverge.Based on the genetic analysis,A.purpurea was considered to be the most closely related species to A.insignis.
Ardea insignisCO Ⅰ geneCyt b genephylogenetic tree
NETL
NSTL
万方数据
