Cloning and Expression Profiling of BR-C Z1 Gene in Helicoverpa armigera
Broad complex(BR-C)has an important role in the growth and development of Helicoverpa armigera.In this study,the cDNA sequence of BR-C Z1 gene was cloned from the midgut of H.armigera larvae based on previous transcrip-tome data,and its amino acid sequence and protein structure were predicted.The fusion protein was expressed by using a prokaryotic expression system and verified by Western blot.Real-time quantitative PCR was used to detect the temporal and spatial expression profiles of BR-C Z1 gene in H.armigera(HaBR-C Z1),and the expression of BR-C Z1 gene in the 6th instar larvae was detected after 2-tridecanone treatment.The results showed that the open reading frame of HaBR-C Z1 gene was 1 284 bp in length,encoding 427 amino acids.The predicted molecular weight and isoelectric point of HaBR-C Z1 were 47.41 kD and 7.72,respectively.Phylogenetic analysis showed that HaBR-C Z1 was closely related to the one of Danaus plexippus plexippus.The relative expression level of HaBR-C Z1 gene was the highest at the pupal stage and in the midgut of the pre-pupa instar larvae.By contrast,the expression of HaBR-C Z1 gene treatment with 20 mg·g-1 of 2-TD peaked(24.7 times as high as that of the control)at 6 h,and then gradually decreased.These results suggested that HaBR-C Z1 might be involved in the growth and development of H.armigera,and respond to 2-TD stimulation.This study contributes to clarifying the expression profiling of HaBR-C Z1 gene and provides a basis for understanding the growth and development mechanism of H.armigera in-depth and pest control.
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