Composition of Pseudois nayaur Gut Bacteria in the Wanglang National Nature Reserve,Sichuan
In order to reveal the composition and influencing factors of blue sheep(Pseudois nayaur)gut microbiota,a to-tal of 53 fresh fecal samples were collected from 5 distribution locations(wild populations)in the Wanglang National Na-ture Reserve(Sichuan Province,China)and the captive population in Chengdu Zoo from April to May 2023.A total of 7 202 473 sequences were obtained from these fecal samples through high-throughput sequencing of PCR products using 16S V3-V4 universal primers.The sequence analysis results showed that Firmicutes(65%)and Bacteroidetes(14%)were the main microbiota in the gut.Microbiota α diversity showed that there was no significant difference between the ob-served species index of the samples from Waicepo and Dacaoping(P>0.05),but which were significantly lower than those from other locations except for Maxionggou(P<0.01).Microbiota β diversity analysis revealed a significant difference be-tween the samples from captive and wild populations.The samples of captive population were enriched with WCHB1_41,Mailhella,and Lysinibacillus.The gut microbiota from Waicepo and Dacaoping,the habitats of which were mainly com-posed of grasslands,were similar.The gut microbiota from Tiebanfang and Jincaopo gathered together,whith less similar-ity.The gut microbiota from Maxionggou,the habitat of which was mainly composed of shrubs,showed a significant varia-tion among individual samples.The LEFSe analysis showed that there were 16 genera that could be used to distinguish wild populations from different locations.Moreover,the gut microbiota of the wild populations from Waicepo were enriched with several opportunistic pathogenic bacteria,such as Parasutterella and Escherichia_Shigella etc.This study provides impor-tant theoretical guidance for the protection of P.nayaur in different locations in the reserve.
Wanglang National Nature ReservePseudois nayaurgut bacteriahabitat
NETL
NSTL
万方数据
维普
