Comparative Study on Genetic Diversity of Parabotia fasciata Populations Based on cyt b Gene Sequence
In order to understand the current status of Parabotia fasciata germplasm resources,the genetic diversity of 9 P.fasciata populations(4 wild populations from Yangtze River and 5 cultured populations)from 7 sites was compared and analyzed based on cyt b gene sequence characteristics.The results showed that a total of 29 haplotypes and 38 polymor-phic loci were detected in 208 samples.The number of haplotypes in the wild populations was 26,while that of the cul-tured populations was only 4.The overall haplotype polymorphism(Hd=0.711)was relatively high,while nucleotide poly-morphism(Pi=0.002 35)was relatively low.The genetic diversity of wild populations(Hd=0.602,Pi=0.003 74)was sig-nificantly higher than that of cultured populations(Hd=0.381,Pi=0.000 91),and this might be due to the cultured popula-tions came from fewer captive-bred parents.The results of neutrality tests,the mismatch peak diagram and Bayesian sky-line plots for the whole wild populations showed a historical expansion.Chishui River population had the most haplotypes and the highest genetic diversity among all the populations(Hd=0.836,Pi=0.008 99),and had large genetic distance and genetic differentiation from other wild populations(0.006 61-0.007 29).These indicated that the current hydrological conditions in the Chishui River were conducive to the hidden habitat and reproduction of P.fasciata.Therefore,Chishui River has a population rich in resources and has formed a stable and relatively independent geographic population with ex-cellent germplasm suitable for future artificial breeding and strain creation.
万方数据
维普
