Screening and Validation of Binding Peptide of Type 2 Bradykinin Receptor from Venomous Gland of Deinagkistrodon acutus
Snake venomous glands contain many small peptides with weak immunogenicity and high biological activities including anti-inflammatory activity.The type 2 bradykinin receptor(B2R)is involved in several important intracellular signaling pathways and is considered as a potential target for anti-inflammatory drug development.In order to obtain spe-cific B2R binding peptides for drug screening,the T7 phage library of Deinagkistrodon acutus venomous gland was used to perform three rounds of biological screening with B2R as the target.Multiple sequences with potential binding ability were obtained.Through a series of bioinformatic analyses such as sequence length,solubility predicting,stability predicting,and molecular docking,a 25 amino acids peptide segment,named DAvp-4,was ultimately determined.The peptide was synthesized,and then the binding ability of DAvp-4 to B2R was measured by surface plasma resonance.The anti-inflammatory effect of DAvp-4 was confirmed in both macrophage cells injured with lipopolysaccharide and diabetic reti-nopathy mouse models.This study obtains a venomous gland peptide with patent medicine potential,but also demonstrates the effectiveness of phage display technology in screening natural product components.
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