摘要
为保护和救治野生金鸻Pluvialis fulva,防止其所携带的条件性致病菌对当地人和畜禽带来健康危害,本试验对陇南腹泻金鹆泄殖腔内细菌进行分离培养、染色镜检、生化试验和16S rRNA基因扩增测序,并对菌株耐药性进行检测.结果鉴定出5株细菌:鸡葡萄球菌Staphylococcus gallinarum(LNJBHl)、大肠埃希氏菌Escherichia coli(LNJBH2)、粪肠球菌Enterococcus faecalis(LNJBH3)、阿氏芽孢杆菌Bacillus aryabhattai(LNJBH4)和费格森埃希菌Escherichia fergusonii(LNJBH5).药敏试验结果显示,5株细菌对林可霉素均耐药,LNJBH1对哌拉西林、氨苄西林、万古霉素、青霉素和红霉素耐药;LNJBH2对头孢唑林、头孢哌酮、氨苄西林、米诺环素、万古霉素、青霉素和红霉素耐药;LNJBH3对卡那霉素、青霉素、庆大霉素和四环素耐药;LNJBH4对头孢他啶、哌拉西林和青霉素耐药;LNJBH5对头孢氨苄、头孢唑林和链霉素耐药,5株细菌对其他抗菌药均敏感或中度敏感.本试验结果为治疗腹泻金鸻提供了理论依据,同时也为陇南当地畜禽养殖防治野生动物源传染病提供了理论基础.
Abstract
To protect and rescue Pluvialis fulva in the wild,and to prevent the spreading of opportunistic pathogens with health risk to local people and livestocks.The cloacal bacteria of P.fulva with diarrhea from Longnan City(Gansu Prov-ince,China)was isolated and cultured,followed by Gram staining,biochemical test,16S rRNA gene sequencing,and an-timicrobial susceptibility testing.The result showed that five strains of bacteria,Staphylococcus gallinarum(LNJBH1),Escherichia coli(LNJBH2),Enterococcus faecalis(LNJBH3),Bacillus aryabhattai(LNJBH4)and Escherichia fergusonii(LNJBH5),were identified.All the tested strains were resistant to lincomycin,while LNJBH1 was resistant to piperacil-lin,ampicillin,vancomycin,penicillin and erythromycin,LNJBH2 was resistant to cefazolin,cefoperazone,ampicillin,minocycline,vancomycin,penicillin and erythromycin,LNJBH3 was resistant to kanamycin,penicillin,gentamicin and tetracycline,LNJBH4 was resistant to ceftazidime,piperacillin and penicillin,LNJBH5 was resistant to cephalexin,ce-fazolin and streptomycin.This study contributes to the treatment of P.fulva with diarrhea and the prevention and control of wild animal infectious disease in livestock and poultry breeding.
基金项目
2023年甘肃省科技计划项目(23JRRK0002)
2023年甘肃省高校教师创新基金项目(2023B-412)
维普
万方数据