Construction and transfer of the plant antisense-expression vector carrying three AP3 homologous genes of Brassica napus
Three AP3 homologous sequences,BnAP3-2、BnAP3-3 and BnAP3-4, were reverse inserted into plasmid pFGC5941,constituted the anti-expression vectors pFGC5941-BnAP3-2,pFGC5941-BnAP3-3 and pFGC5941-BnAP3-4. The vectors were introduced into the grobacteriun strain EHA105 by means of rapid frozen thaw method and transferred into hypocotyls of Brassica napus. Thirty one resistant plants with antisense BnAP3-2 gene, twenty resistant plants with antisense BnAP3-3 gene and forty two resistant plants with antisense BnAP3-4 gene were obtained from the MS medium with 5 mg/L PPT. Twelve transgenic plants with antisense BnAP3-2 gene, seven transgenic plants with antisense BnAP3-3 gene and sixteen transgenic plants with antisense BnAP3-4 gene were identified by PCR. Semi-quantitative PCR analysis confirmed that antisense fragment of the there foreign AP3 homologous genes had been expressed in the B. napus plants.
国家科技期刊平台
NSTL
万方数据
维普
