A transcriptional regulatory network of Bacillus pumilus predicted by comparative genomcis methods
To explore the gene transcriptional regulatory relations of Bacillus pumilus, a candidate tran-scriptional regulatory network of B. Pumilus has been predicted by searching the homologous transcription factors (TFs) and the homologous regulated genes from Bacillus subtilis genome. The positional weight matrix(PWM) of transcription factor binding sites(TFBSs) was used to scan the whole B. Pumilus genome in order to verify the candidate transcriptional regulatory networks. Finally, a more complete transcriptional regulatory networks had been constructed by integrating with other B. Pumilus TFs and regulated genes. The network consist of 195 TFs and 1201 regulated genes. The result showed that, in bacteria, with the data set of the closely related model species which has a long history of experimental research, it is feasible to predict the transcriptional regulatory network by using comparative genomics methods. The study provided a feasible approach for exploring gene regulatory networks of the organism which lacks large-scale gene expression microarray data.
国家科技期刊平台
NSTL
万方数据
维普
