Preliminary study on the Davidia involucrata anther callus induction
The anthers of the endangered plant Davidia involucrata were chosed as explants for the first time to induce anther callus in this study. The relationship between bud appearances and developmental stages of microspores was identified first. And the anthers were cultured in MS mediums contain different hormone types, concentrations and sugar concentrations. The result indicated that the effect of low-temperature pretreatment on callus is not significant. The callus inductivity is decreased gradually with the extending of low-temperature treatment time. It also indicated that high concentration of sugar is bad for the formation of callus. The best medium to induce anther callus in this study is using 1. 7~2. 2 cm long bud, 4 ℃ pretreated 2 day and the medium MS4-2,4-D 1 mg/L+NAA 0. 5 mg/L+GA3 lmg/L + 5% sugar; the highest inductivity is 91. 3. After the callus induction, some explorations of the induction of bud had been done. However, due to the serious browning of callus, the differentiation was unsuccessful.
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