Uraniuminduced effects on development and antioxidase of Brassica napus L.
This research aimed to study effects on growing development and alterations in transcriptional and protein level of some ROS-scavenging enzymes for Brassica napus seedlings following uranium exposure.The results showed that compared with control group,fresh weight,height and germination rate of Brassica napus seedlings did not decrease with increasing uranium stress concentration,but the root length was significantly affected as the treatment of elevated concentrations and suppression time.Uranium exposure caused an obvious change in ion leakage and lipid peroxidation,significantly increasing has been observed in relative electrical conductivity(120.6％) and content of malondialdehyde(about 25 times).Analysis of ROS-scavenging enzymes capacities as SOD,POD and CAT showed sharply decreased when concentration and time of uranium contamination stress were increased.Changes in gene expression for different ROS-scavenging enzymes as CAT1,APX and CSD1 has been analysed,and transcript levels of CAT1 and APX were significantly down-regulated under any uranium concentration.Interestingly,the expression of CSD1 was up-regulated under low uranium concentration,but its expression was down-regulated when exposed to high uranium concentration.
国家科技期刊平台
NSTL
万方数据
维普
