Studies on the mechanism of Stellera chamaejasme L.to Locusta migratoria manilensis (Meyen)
Using silica gel column to separate the ethyl acetate extract of Stellera chamaejasme L.we can get Ⅰ,Ⅱ,Ⅲ sections.The activities of Na+-K+-ATPase、Ca2+/Mg2+-ATPase and acetylcholinesterase of insects were determined in vivo.The results showed that Ⅰ section from S.chamaejasme L.had the strongest poison effect on Locusta migratoria manilensis (Meyen).Under the concentration of 10 mg/ mL,the corrected mortality was 92.50％ in 72 hour.And the poison effect there was persistence.The Ⅰ section from S.chamaejasme L.also had certain inhibition on activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase.The inhibiton rate of Na+-K+-ATPase was the highest among the three kinds of enzymes.There was a well correlation between the toxic action of the active ingredients from S.chamaejasme L.to L.migratoria manilensis (Meyen) and the inhibition on activity of Na+-K+-ATPase in L.migratoria manilensis (Meyen).Na+-K+-ATPase is likely the target of the active ingredients from S.chamaejasme L.against L.migratoria manilensis (Meyen).
国家科技期刊平台
NSTL
万方数据
维普
