Analysis of the part characteristics and the difference of amylase in tuberous roots of sweet potato during storage period
The part characteristics and the difference of amylase were investigated in tuberous root of sweet potato ,during storage period .The total soluble protein were extracted with 3 mL /g buffer ,it harvests between 2 weeks in the storage time .Difference amylases were found in isozyme composition in tuberous roots of sweet potato during storage period ,by electrophoresis on 8％ native polyacrylamide gel and active stain by using soluble starch as substrate .New amylase responds differently to inhibitors , it is sensitive to EDTA or thiol-group reagent ,and it is more sensitive to temperature than β-amylase . New amylase can be metalloenzyme with disulfide bond .
国家科技期刊平台
NSTL
万方数据
维普
