为使用生物信息学系统分析孕激素相关子宫内膜蛋白(Progestagen-associated endo-metrial protein，PAEP)在胃癌中的早期诊断和预后预测性能及免疫浸润相关性，该研究构建预测模型ROC曲线和偏差校正曲线检验其性能准确性，并通过细胞实验进一步验证分析结果。生信分析结果显示，与正常组织相比，胃癌组织中PAEP的表达较高，在早期(T1期、N0期、M0期)即有统计学意义(P＜0。001)，且诊断胃癌的准确性较高(ROC曲线下面积为0。889);PAEP高表达的胃癌患者预后较差(P≤0。001);TNM分期越晚，年龄越大，PAEP表达越高，胃癌患者生存概率越低，偏差校正曲线接近理想曲线(45°线)，显示预测结果良好;PAEP的表达与胃癌中的中性粒细胞、巨噬细胞的浸润水平呈正相关趋势，与B细胞、中央记忆型T细胞、T细胞呈显著的负相关趋势。qRT-PCR和 Western blot结果显示，HGC-27、BGC-823、MKN-45、SGC-7901和AGS细胞中PAEP基因转录水平和蛋白表达水平均显著高于GES-1。结果表明了 PAEP蛋白可用于胃癌诊断和预后的生物标志物，并进行了实验验证，其机制与免疫有关。
The role of Progestagen-associated endometrial protein in early diagnosis and prognosis of gastric cancer
To use a bioinformatics system to analyze the early diagnosis and prognosis prediction per-formance and immune infiltration correlation of Progestagen-associated endometrial protein(PAEP)in gastric cancer,this study constructed a predictive model ROC curve and deviation correction curve to test its performance accuracy,and further validate the analysis results through cell experiments.The re-sults of bioinformatics analysis showed that the expression of PAEP in gastric cancer tissues was higher than that in normal tissues,which was statistically significant in the early stages(T1,N0,M0)(P＜0.001),and the diagnostic accuracy of gastric cancer was higher(the area under ROC curve was 0.889).The prognosis of gastric cancer patients with high expression of PAEP was poor(P≤0.001).The later the TNM stage,the older the age,the higher the expression of PAEP,and the lower the sur-vival probability of gastric cancer patients,and the deviation correction curve was close to the ideal curve(45 ° line),indicating a good predictive result.The expression of PAEP was positively correlated with the infiltration level of neutrophils and macrophages in gastric cancer,and negatively correlated with B cells,central memory T cells and T cells.qRT-PCR and Western blot results showed that the transcrip-tional and protein expression levels of PAEP in HGC-27,BGC-823,MKN-45,SGC-7901 and AGS cells were significantly higher than those in GES-1 cells.The results indicate that PAEP protein can be used as a biomarker in the diagnosis and prognosis of gastric cancer,which is verified by experiments,and its mechanism is related to immunity.
国家科技期刊平台
NSTL
万方数据
维普
