成骨细胞线粒体移植促骨髓间充质干细胞体外增殖、迁移和成骨分化
Mitochondrial transplantation of osteoblasts promotes proliferation,migration and osteogenic differentiation of bone marrow mesenchymal stem cell in vitro
杨雅丽 1司琪琦 2王思瑜 2周嘉裕 2郭泰林3
作者信息
- 1. 西南交通大学生命科学与工程学院, 成都 610031;西南交通大学医学院, 成都 610031
- 2. 西南交通大学生命科学与工程学院, 成都 610031
- 3. 西南交通大学医学院, 成都 610031
- 折叠
摘要
为探究成骨细胞(OBs)线粒体移植对骨髓间充质干细胞(BMSCs)向成骨分化的影响,本研究体外人工提取BMSCs线粒体和OBs线粒体,分别移植入受体BMSCs.实验结果发现,接受成骨细胞线粒体的BMSCs表现出显著增强的增殖、迁移、抗凋亡和成骨能力.此外,接受成骨细胞线粒体的BMSCs胞内活性氧水平降低、氧气消耗速率上调、ATP产量增加.以上结果表明,(1)成骨细胞线粒体移植能够增强体外BMSCs功能,促进其向成骨分化;(2)相较于接受骨髓间充质干细胞线粒体,接受成骨细胞线粒体移植的受体BMSCs具有更强的有氧代谢能力以及更强的成骨分化能力,有望为骨生长与骨损伤修复提供一种候选方法.
Abstract
To explore the effect of mitochondrial transplantation of osteoblasts(OBs)on osteogenic differen-tiation of bone marrow mesenchymal stem cells(BMSCs),this study artificially extracted BMSCs mitochon-dria and OBs mitochondria in vitro and transplanted them into recipient BMSCs respectively.The experimen-tal results showed that BMSCs receiving osteoblast mitochondria showed significantly enhanced proliferation,migration,anti-apoptosis and osteogenesis.In addition,the level of intracellular ROS in BMSCs receiving os-teoblast mitochondria decreased,the rate of oxygen consumption increased,and ATP production increased.These results indicated that:(1)Mitochondrial transfer can enhance the function of BMSC in vitro and pro-mote its osteogenic differentiation;(2)Compared with bone marrow mesenchymal stem cell mitochondria,recipient BMSCs receiving osteoblast mitochondrial transplantation may have stronger aerobic metabolic ca-pacity and osteogenic differentiation ability,which is expected to provide a candidate method for bone growth and bone injury repair.
关键词
线粒体移植/骨髓间充质干细胞/成骨细胞/成骨分化/代谢Key words
Mitochondria transplantation/Bone marrow mesenchymal stem cells/Osteoblasts/Osteogenic differentiation/Metabolism引用本文复制引用
基金项目
四川省科技厅应用基础研究项目(Q114321S01003)
出版年
2024
国家科技期刊平台
NSTL
万方数据