Cloning and functional identification of AP2/ERF family-related genes in Davidia involucrata Baill.
To investigate the role of AP2/ERF transcription factors in the regulation of Dove tree flowering and floral organ development,this study was conducted to screen the AP2/ERF family genes DiAP2-1,Di-DREB-1 and DiRAV-1 from the transcriptomic data of dove bracts and leaves at different developmental stages in the laboratory.A preliminary study of the regulatory mechanisms of these genes in the development of dove bracts was conducted by bioinformatics analysis,gene cloning and functional characterization.Subcel-lular localization experiments showed that DiDREB-1 and DiRAV-1 were expressed in the nucleus,except for DiAP2-1,which was mainly expressed in the cytoplasm.Analysis of the expression pattern of the target genes in dove showed that the expression levels of DiAP2-1 and DiRAV-1 gradually decreased during bract development,while DiDREB-1 was only highly expressed during the third bract period,presumably Di-DREB-1 and DiRAV-1 belonged to class A genes and DiAP2-1 belonged to class B genes.Phenotypic ex-periments showed that DiDREB-1 and DiRAV-1 transgenic Arabidopsis strains exhibited early flowering compared to the wild type,and the Arabidopsis mutant corresponding to the DiAP2-1 homologue showed re-duced petals and larger sepals,and short,stubby and failure to breed hornbeam compared to the wild type Arabidopsis at the same time.These results suggest that the AP2/ERF-like transcription factors DiAP2-1,DiDREB-1 and DiRAV-1 play key roles in the development of dove bracts and flowering organs and in the regulation of Dove tree flowering.
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