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ASI基因家族结构及表达分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
为研究编码α-淀粉酶/枯草杆菌蛋白酶抑制剂(α-amylase/subtilisin inhibitor，ASI)的基因结构特征及功能，本研究利用NCBI、Phytozome等平台工具鉴定到来源于23种植物的33个ASI基因家族成员，分析其进化特点及其编码蛋白的理化性质和保守基序等信息。同时结合RT-qPCR技术分析水稻ASI在干旱和盐胁迫条件下的表达模式。结果表明，ASI基因家族具有较高的保守性，但其编码蛋白上下游区域的保守性强弱不一，同时顺式作用元件预测结果提示ASI基因可能参与植物生长发育调控、响应生物及非生物胁迫等生理过程。RT-qPCR分析发现，较未胁迫条件下，幼苗期水稻的根、叶鞘和叶片等组织中RASI基因发生了不同的表达变化，提示ASI基因可能具有多样性的生物学功能。
Structural and expression analysis of the ASI gene family
To study the structural features and functions of genes encoding α-amylase/subtilisin inhibitor(ASI),a total of 33 ASI gene family members from 23 plant species were identified in this study by using NCBI,Phytozome and other platforms.The evolutionary characteristics the physicochemical properties and conserved motifs of ASI proteins were analyzed.At the same time,RT-qPCR was combined to analyze the expression pattern of RASI under drought and salinity stress.The results showed that the ASI gene family is highly conserved,but the upstream and downstream regions of the encoded proteins are differently conserved.Meanwhile,the cis-acting element prediction results suggested that the ASI genes may be involved in the physiological processes such as the regulation of plant growth and development,and the response to biotic and abiotic stresses.RT-qPCR analysis revealed that the expression of RASI in roots,leaf sheaths and leaves of rice at the seedling stage changed differently compared with the unstressed condition.These results indicate that ASI genes may have diverse biological functions.

α-amylase/subtilisin inhibitor,ASIKunitz-domain inhibitorsGene family identificationGene sequences analysisGene expression analysis

黄斌翰、何艳艳、唐婕、叶春、周嘉裕、廖海

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西南交通大学生命科学与工程学院, 成都 610031

α-淀粉酶/枯草杆菌蛋白酶抑制剂(ASI) Kunitz型抑制剂 基因家族鉴定 基因序列分析 基因表达分析

国家自然科学基金成都市科技局项目中央高校医工结合项目中央高校医工结合项目

322704102022-YF05-01357-SN2682021ZTPY0172682022ZTPY053

2024

10.19907/j.0490-6756.2024.026003

四川大学学报(自然科学版)
四川大学

四川大学学报(自然科学版)

CSTPCD北大核心
影响因子：0.358
ISSN：0490-6756
年,卷(期)：2024.61(2)
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