ASI基因家族结构及表达分析
Structural and expression analysis of the ASI gene family
黄斌翰 1何艳艳 1唐婕 1叶春 1周嘉裕 1廖海1
作者信息
- 1. 西南交通大学生命科学与工程学院, 成都 610031
- 折叠
摘要
为研究编码α-淀粉酶/枯草杆菌蛋白酶抑制剂(α-amylase/subtilisin inhibitor,ASI)的基因结构特征及功能,本研究利用NCBI、Phytozome等平台工具鉴定到来源于23种植物的33个ASI基因家族成员,分析其进化特点及其编码蛋白的理化性质和保守基序等信息.同时结合RT-qPCR技术分析水稻ASI在干旱和盐胁迫条件下的表达模式.结果表明,ASI基因家族具有较高的保守性,但其编码蛋白上下游区域的保守性强弱不一,同时顺式作用元件预测结果提示ASI基因可能参与植物生长发育调控、响应生物及非生物胁迫等生理过程.RT-qPCR分析发现,较未胁迫条件下,幼苗期水稻的根、叶鞘和叶片等组织中RASI基因发生了不同的表达变化,提示ASI基因可能具有多样性的生物学功能.
Abstract
To study the structural features and functions of genes encoding α-amylase/subtilisin inhibitor(ASI),a total of 33 ASI gene family members from 23 plant species were identified in this study by using NCBI,Phytozome and other platforms.The evolutionary characteristics the physicochemical properties and conserved motifs of ASI proteins were analyzed.At the same time,RT-qPCR was combined to analyze the expression pattern of RASI under drought and salinity stress.The results showed that the ASI gene family is highly conserved,but the upstream and downstream regions of the encoded proteins are differently conserved.Meanwhile,the cis-acting element prediction results suggested that the ASI genes may be involved in the physiological processes such as the regulation of plant growth and development,and the response to biotic and abiotic stresses.RT-qPCR analysis revealed that the expression of RASI in roots,leaf sheaths and leaves of rice at the seedling stage changed differently compared with the unstressed condition.These results indicate that ASI genes may have diverse biological functions.
关键词
α-淀粉酶/枯草杆菌蛋白酶抑制剂(ASI)/Kunitz型抑制剂/基因家族鉴定/基因序列分析/基因表达分析Key words
α-amylase/subtilisin inhibitor,ASI/Kunitz-domain inhibitors/Gene family identification/Gene sequences analysis/Gene expression analysis引用本文复制引用
基金项目
国家自然科学基金(32270410)
成都市科技局项目(2022-YF05-01357-SN)
中央高校医工结合项目(2682021ZTPY017)
中央高校医工结合项目(2682022ZTPY053)
出版年
2024
国家科技期刊平台
NSTL
万方数据