首页|响应面优化超声波微波协同提取猕猴桃茎黄酮工艺及抗氧化活性分析

响应面优化超声波微波协同提取猕猴桃茎黄酮工艺及抗氧化活性分析

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
为提高软枣猕猴桃树体资源利用率，以野生软枣猕猴桃茎为实验原料，并使用超声波微波协同提取猕猴桃茎总黄酮，采用Plackett-Burman试验设计，评价影响茎黄酮提取效率的超声功率、超声时间等7个因素，并筛选出其中3个显著影响因素:料液比、超声温度、微波时间。在此基础上，进一步采用Box-Benhnken最陡爬坡路径方法及响应面设计优化出3个显著影响因素的最佳参数水平。结果显示:料液比45 mL/g、超声温度55℃、微波时间5。5 min，此条件作用下，黄酮类化合物提取率可达9。25%。在测定的质量浓度中，软枣猕猴桃茎黄酮对DPPH、羟基的清除能力优于VC，而对超氧阴离子的清除差于VC，但对三种自由基均有一定清除能力。以上结果表明，软枣猕猴桃茎黄酮提取物具有良好的抗氧化活性。
Response surface optimization of ultrasonic-microwave synergistic extraction of flavonoids from Actinidia arguta stems and analysis of antioxidant activity
In order to enhance the utilization efficiency of Actinidia arguta tree resources,the stems of wild Actinidia arguta were used as experimental raw materials for the extraction of total flavonoids using ultrasonic-microwave synergistic extraction.The Plackett-Burman experimental design was employed to evaluate the ultrasonic power and ultrasonic time affecting the extraction efficiency of flavonoids from stems.Seven factors,including ultrasonic power and ultrasonic time,were evaluated,and three of them were se-lected as significant influencing factors:solid-liquid ratio,ultrasonic temperature,and microwave time.On this basis,the Box-Benhnken steepest ascent path method and response surface design were further used to optimize the optimal parameter levels of the three significant influencing factors.The results showed that the extraction rate of flavonoids could reach 9.25%under the conditions of a solid-liquid ratio of 45 mL/g,ultra-sonic temperature of 55 °C and microwave time of 5.5 min.In the measured mass concentration,the scaveng-ing ability of soft jujube kiwi stem flavonoids on DPPH and OH was better than that of VC,while the scav-enging ability of O2-was lower than that of VC,but it had certain scavenging ability for three free radicals.The results showed that the flavonoid extract of Actinidia arguta stem had good antioxidant activity.

Actinidia arguta stemUltrasonic microwave technologyFlavonePlackett-Burman designResponse surface methodAntioxidation

姜立春、张雨洁、蒋道玉、付丹阳、赵欣悦

展开 >

绵阳师范学院生命科学与技术学院,绵阳 621000

软枣猕猴桃茎 超声波微波技术 黄酮 Plackett-Burman试验设计 响应面法 抗氧化

四川省科技厅自然科学研究项目阿坝州科知局应用技术研究与开发资金项目

2023NSFSC020619YYJSYJ0031

2024

10.19907/j.0490-6756.2024.036002

四川大学学报(自然科学版)
四川大学

四川大学学报(自然科学版)

CSTPCD北大核心
影响因子：0.358
ISSN：0490-6756
年,卷(期)：2024.61(3)