Target proteins analysis and validation of NGR1 on inflammatory response in Diabetic nephropathy
This study aimed to investigate the molecular mechanism and potential targets of Zhuang Medicine Panax-Notoginseng NGR1 on the inflammatory response in Diabetic nephropathy(DN).Targets of NGR1 were found by the Swiss Target Prediction database.Genes related to inflammatory response in DN were re-trieved from OMIM and Gene Cards databases.A protein-protein interaction(PPI)network between the above intersection targets was constructed.GO and KEGG pathway enrichment analysis was conducted,fol-lowed by molecular docking analysis of NGR1 with its targets by using Autodock Vina.The affinity between NGR1-targets related to the inflammatory response in DN and NLRP3 inflammasome was evaluated by GRAMM-X.MPC-5 cells were used for in vitro detection.The results showed that STAT3 and VEGFA were the main targets of NGR1,which were closely related to NLRP3.PI3K/Akt signaling pathway,etc.was involved in NGR1 protection against DN.Cell experiments showed that NGR1 reduced the expression levels of P-STAT3,VEGFA and NLRP3.The results suggest that NGR1 interfered with the activation of NLRP3,regulated the expression of VEGFA and then prevented DN,through lower-down the phosphorylation level of STAT3.
国家科技期刊平台
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