Determination of Anthocyanin-type Active Substances Contents in Tea by HPLC-MS/MS Method
Anthocyanin,as one of the major bioactive components in tea,represents the edible value.Due to the enrichment of tea in protein,sugar,tea polyphenols,flavonoids,alkaloids,minerals,vitamins,pigments and other components,it is difficult to determine the anthocyanin active substances in tea matrix by relevant domestic standards.Thus,this paper established a high-performance liquid chromatography-tan-dem mass spectrometry(HPLC-MS/MS)method for the detection of six anthocyanin compounds in tea sample under the optimization of pretreatment conditions and instrument conditions.The established meth-od showed a good linear relationship within the concentration range of 25 to 2500 μg/L,with correlation coefficients(R)greater than 0.99,recovery rates ranging from 91.95%to 106.99%,and precision RSD less than 10%,which meet the requirements for analytical performance.The method exhibited advantages such as easy operation,high sensitivity,and high accuracy.These advantages make it suitable for related analyt-ical detections and providing valuable guidance for the establishment of standard methods for anthocyanin content detection in tea.
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