Chiral Separation of Three 1,4-dihydropyridine Drugs by Aqueous Two-phase Chiral Extraction
A method was established for the separation of three 1,4-dihydropyridine chiral enantiomers using ethanol/ammonium sulfate as a biphasic system.Single factor experiments were conducted to investigate the effects of the type of chiral recognition agent,recognition agent concentration,concentration of the components to be separated,pH value,and other factors.The optimal separation conditions were determined as follows:system pH value of 3,ME-β-CD concentration of 0.3 mmol/L,L-tartrate octyl ester concentration of 0.4 mmol/L,and three 1,4-dihydropyridine drug concentrations of 0.4 mmol/L.Three 1,4-dihydropyridine drug enantiomers were separated using 0.1％triethylamine n-hexane-0.1％triethylamine ethanol(90:10)as the mobile phase,and the detection limits,linear ranges,and recovery rates of the three drug enantiomers were investigated.The results showed that the detection limits of the enantiomers of the three drugs were between 0.020～0.035 mg/L,and the correlation coefficients were not less than 0.999.The linear relationship of the method was good,with a recovery rate of 92.2％～105.3％and a relative standard deviation of 0.53％～1.87％.This method is accurate,simple,and can be used for the separation and content determination of three drugs and their formulations.
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