A intermittence method was adopted to study the adsorption of wood fiber for Sr2+ and Cs+ under various conditions including different adsorbent size, pH, media, adsorption time, temperature and dosage of adsorbent, and mechanisms for Sr2+ and Cs+ removal were discussed by FTIR and energy spectrum analysis.When 0.2 g wood fiber dissolved in 50 mL solution, with Sr2+ and Cs+ concentration 0.5 mmol· L-1, pH=5.0, then batch adsorpted respectively for 120 min and 60 min at room temperature, the removal rate of Sr2+ achieved 82.4%, while Cs+ was 25%.The biosorption kinetics can be described by the pseudo second-order equation quite well (R2=0.999 9 and 1.000).FTIR analysis indicated that hydroxyl, hydrogen bond, carbon-carbon bond and methylene groups were the main functional groups in the reaction with Sr2+ and Cs+.Energy spectrum analysis indicated that wood fiber adsorption process of uranium ions existed in ion exchange.Therefore, there was physical adsorption and chemical adsorption existing in the adsorption process, which adsorption types were mixed.